Abstract: In response to China's land refinement transformation, improving the spatio-temporal accuracy of land use evolution detection and clarifying the spatio-temporal heterogeneity of land use are of great significance for regional coordinated and sustainable development.Using land transfer matrix and ecological quality model, this paper discusses the spatial evolution of land use and ecological quality in Shandong Province from 1990 to 2020.The results showed that:(1) The land use structure changed significantly, mainly cultivated land, construction land, water area and forest land.The net increase of area mainly includes construction land and water area, and the net decrease of area mainly includes grassland, land, unused land and forest land.(2) The area ratio of high-value areas of land-use ecological quality continued to rise and then decline, while the area ratio of low-value areas continued to decline and then rise.High ecological value areas gradually change from aggregation to separation, and low ecological quality areas gradually change from separation to aggregation.