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    稀释法对大豆脲酶提取效率及固化效果的影响

    Impact of Dilution Technique on the Efficiency of Soybean Urease Extraction and Solidification Effect

    • 摘要: 酶诱导碳酸钙沉淀技术(EICP)环境友好,被广泛应用于多种土壤改良.然而,在现场试验中,脲酶提取耗时较长且离心成本较高.为解决这一问题,采用实验室内提取高浓度脲酶溶液后稀释的方法,通过溶液试验、X射线衍射(XRD)与扫描电子显微镜(SEM)测试,对比分析了初始浓度为100g/L的大豆脲酶溶液稀释至20g/L与直接制备20g/L溶液的提取效率以及沉淀物的微观结构.随后进行了无侧限抗压强度(UCS)测试和碳酸钙质量分数测试,以分析这两种处理方式在固化效果上的差异.试验结果表明:将脲酶溶液稀释至等效浓度后,尽管初始浓度不同,但脲酶活性、碳酸钙产率以及无侧限抗压强度没有显著差异;稀释并未改变碳酸钙的矿物成分和晶型,其表面分布着球状和类球状晶体;等效原料浓度为60g/L时,试样经EICP固化后,无侧限抗压强度最高,碳酸钙分布最均匀.从宏观和微观的角度看,采用离心后稀释的方法可显著提高脲酶的提取效率,进而改善EICP技术的经济效益.

       

      Abstract: Enzyme-Induced Calcium Carbonate Precipitation (EICP) technology,renowned for its environmental amiability,is extensively employed in the amelioration of various soils.However,in field trials,the extraction of urease proves time-intensive and incurring substantial centrifugation costs.To redress this issue,an approach of diluting high-concentration soybean urease solution extracted within laboratory confines was embraced.Through solution assays,X-ray diffraction (XRD),and scanning electron microscopy (SEM) investigations,a comparative scrutiny ensued between the extraction efficiency from an initial concentration of 100g/L of soybean urease solution diluted to 20g/L and the direct preparation of a 20g/L solution,alongside a microscopic evaluation of precipitate structures.Subsequent unconfined compressive strength (UCS) assessments and analysis of calcium carbonate mass fractions were conducted to discern the divergences in solidification efficacy between the two methodologies.Experimental findings elucidate that despite disparate initial concentrations,there exist no pronounced disparities in urease activity,calcium carbonate yield,or unconfined compressive strength subsequent to diluting the urease solution to equivalent concentrations.Dilution,in essence,does not alter the mineral composition or crystalline configuration of calcium carbonate,which is characterized by the presence of spherical and quasi-spherical crystals upon its surface.At an equivalent raw material concentration of 60g/L,the unconfined compressive strength values post EICP solidification peak,accompanied by a most uniform distribution of calcium carbonate.From both macroscopic and microscopic vantage points,adoption of the post-centrifugation dilution method conspicuously augments the efficiency of urease extraction,thereby ameliorating the economic viability of EICP technology.

       

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