Abstract:
Enzyme-Induced Calcium Carbonate Precipitation (EICP) technology,renowned for its environmental amiability,is extensively employed in the amelioration of various soils.However,in field trials,the extraction of urease proves time-intensive and incurring substantial centrifugation costs.To redress this issue,an approach of diluting high-concentration soybean urease solution extracted within laboratory confines was embraced.Through solution assays,X-ray diffraction (XRD),and scanning electron microscopy (SEM) investigations,a comparative scrutiny ensued between the extraction efficiency from an initial concentration of 100g/L of soybean urease solution diluted to 20g/L and the direct preparation of a 20g/L solution,alongside a microscopic evaluation of precipitate structures.Subsequent unconfined compressive strength (UCS) assessments and analysis of calcium carbonate mass fractions were conducted to discern the divergences in solidification efficacy between the two methodologies.Experimental findings elucidate that despite disparate initial concentrations,there exist no pronounced disparities in urease activity,calcium carbonate yield,or unconfined compressive strength subsequent to diluting the urease solution to equivalent concentrations.Dilution,in essence,does not alter the mineral composition or crystalline configuration of calcium carbonate,which is characterized by the presence of spherical and quasi-spherical crystals upon its surface.At an equivalent raw material concentration of 60g/L,the unconfined compressive strength values post EICP solidification peak,accompanied by a most uniform distribution of calcium carbonate.From both macroscopic and microscopic vantage points,adoption of the post-centrifugation dilution method conspicuously augments the efficiency of urease extraction,thereby ameliorating the economic viability of EICP technology.